FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5991202
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT101318116.911147379106897Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT107050.17867866915520458Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT99520.16611023964807062Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC93630.15627915733770953RNA PCR Primer, Index 16 (96% over 25bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA91170.1521731365425502No Hit
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC90130.15043725783240158RNA PCR Primer, Index 16 (96% over 25bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT79310.13237744279027813Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA76890.1283381865608938No Hit
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC70750.11808982571443927Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT69490.11598674189252842Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAT64580.10779139144365354No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA1170250.0138.875981
CTGCAAT1169700.0138.061832
CCGCAAT157700.0134.373412
TGCAATT1340200.0131.136963
ACCGCAA162300.0130.74951
CGCAATT170900.0129.64713
GCAATTT1861900.0107.0613564
CAATTTC1876000.0106.736865
AATTTCC1886050.0106.64146
TTTCCAC1875850.0105.8145758
ATTTCCA1895150.0105.27257
TTCCACA1906150.0104.394979
CGGCAAT22300.095.123242
ACGGCAA27000.078.864141
TATGCAA47900.077.8318941
GGCAATT29700.076.558413
ATGCAAT140600.067.323662
ATTGCAA52450.066.92331
GCAATTC40850.052.105144
GATTGGA39350.052.0610582
Produced by FastQC (version 0.11.5)