FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14709447
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT6069844.126490955098448Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA1749200.0134.176281
CTGCAAT1739050.0134.171572
TGCAATT1972750.0128.067463
CCGCAAT248200.0126.688322
ACCGCAA250100.0125.991751
CGCAATT262350.0125.557933
GCAATTT2653350.0108.4780044
AATTTCC2713100.0107.0297556
CAATTTC2704050.0106.9330755
TTTCCAC2729300.0105.029258
ATTTCCA2769800.0104.048387
TTCCACA2767300.0103.8568349
CGGCAAT44350.077.648992
ACGGCAA54300.063.5711821
ATGCAAT226250.054.9430162
GGCAATT67500.052.524243
ATTGCAA126400.052.4341551
TATGCAA86000.041.9130941
CATGCAA107050.039.305961
GCAATTC90450.036.2252734
Produced by FastQC (version 0.11.5)