FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10564022
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT2238722.119192860446523Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1123650.0132.32942
ACTGCAA1133150.0131.877671
TGCAATT1273600.0126.77823
ACCGCAA153650.0126.303191
CGCAATT162100.0124.7510453
CCGCAAT155450.0124.526452
GCAATTT1709650.0107.223414
CAATTTC1748450.0105.371815
AATTTCC1759150.0105.2422566
TTTCCAC1770750.0103.324688
TTCCACA1792650.0102.183729
ATTTCCA1801500.0102.100237
CGGCAAT27550.073.850692
ACGGCAA37650.054.82631
ATGCAAT152150.050.4324532
ATTGCAA91050.046.2204551
TCCGATC30000.041.1757665
GGCAATT53850.038.7266733
TTCCGAT33900.035.581354
CCGATCT36700.033.184286
Produced by FastQC (version 0.11.5)