FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7764951
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT5487657.067204931492807Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA78120.10060591496327535No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA985700.0134.876081
CTGCAAT983750.0134.589772
CCGCAAT133800.0130.116242
ACCGCAA136250.0128.504641
TGCAATT1145250.0126.431643
CGCAATT149100.0125.2901153
GCAATTT1572400.0104.6594244
CAATTTC1593500.0103.797975
AATTTCC1608250.0103.428616
TTTCCAC1607300.0102.344178
TTCCACA1627950.0101.139489
ATTTCCA1637800.0100.8720257
CGGCAAT22050.085.3477252
ACGGCAA29100.065.187751
ATTGCAA74000.059.8141751
ATGCAAT138350.049.78862
GGCAATT39150.048.810853
TATGCAA47700.043.577891
TCGCAAT13600.041.1388932
ACGCAAT19500.040.98822
Produced by FastQC (version 0.11.5)