FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2500701
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT103789441.50412224412275Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT307241.2286154962148612Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT218640.87431484211827Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT179900.7193982807220856Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT162970.6516972640871499Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT118750.474866847336007Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC89880.3594192188510342RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC88120.3523811923136752RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC69350.2773222388442281Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT54480.217858912360974Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGACACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC47360.1893868959143856RNA PCR Primer, Index 16 (100% over 22bp)
AGATCGGAAGAGCACACGTCTTAACTCCAGTCCATCTCGTATGCCGTCTT45410.18158908242128907Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC42810.17119199776382701Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC42480.16987236778807224TruSeq Adapter, Index 16 (95% over 22bp)
AGATCGGAAGAGCAACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC40840.1633142066964423TruSeq Adapter, Index 16 (95% over 22bp)
AGATCGGAAGAGCACAGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC35040.14012071015287314TruSeq Adapter, Index 16 (95% over 22bp)
AGATCGGAAGAGCACACTTCTGAACTCCAGTCCATCTCGTATGCCGTCTT34110.13640175294847323Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCTGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT32800.13116322183259815Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCTTAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT32730.13088330032258955Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
NGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT30250.12096608111085651Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGTAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT28740.11492777425209971Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAGCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT27190.10872951224476658Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC26820.10724992712043543Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA247550.0134.241241
CTGCAAT250900.0131.955052
TGCAATT310000.0124.968463
GATTGGA42200.0117.843952
AGATTGG43350.0116.672321
CGCAATT38500.0115.612113
CCGCAAT36700.0111.801092
ATTGGAA45650.0109.25553
GACCGGA34050.0109.218432
AGACCGG34100.0108.8965761
TTGGAAG47750.0104.146924
AATTTCC458250.0102.577856
ACCGGAA36150.0102.472713
CAATTTC448950.0101.905095
GCAATTT429900.0101.7160644
ATTTCCA465800.0100.617487
TTTCCAC470200.099.2750558
CCGGAAG38300.096.720344
ACCGCAA43400.094.770771
TTCCACA497450.093.9679959
Produced by FastQC (version 0.11.5)