FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000117
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT214404753.599607211489065Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT635661.5891035187220774Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT453111.1327418673003815Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT370560.92637290359257Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT327560.8188760478755996Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT241340.6033323525286886Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC178010.44501198339948556RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC176950.44236206090971836RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC138520.3462898710212726Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT109510.2737669923154748Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGCACACGTCTTAACTCCAGTCCATCTCGTATGCCGTCTT101650.25411756706116345Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGACACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC93840.2345931381507091RNA PCR Primer, Index 16 (100% over 22bp)
AGATCGGAAGAGCAACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC85070.21266877943820145TruSeq Adapter, Index 16 (95% over 22bp)
AGATCGGAAGAGACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC84930.212318789675402TruSeq Adapter, Index 16 (95% over 22bp)
AGATCTTAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT72290.180719713948367Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
AGATCTGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT72180.18044472199188175Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGCACAGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC70720.17679482875125901TruSeq Adapter, Index 16 (95% over 22bp)
AGATCGGAAGAGCACACTTCTGAACTCCAGTCCATCTCGTATGCCGTCTT69670.17416990553026324Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC66130.16532016438519173Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
NGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT66020.16504517242870645Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC63290.1582203720541174Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAGCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT63080.15769538740991826Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGTAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT62670.15667041739029133Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC47670.11917151423320868Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGGAAGGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC44480.11119674749513576RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAAGAGCACACTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC44020.1100467811316519RNA PCR Primer, Index 16 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA284450.0128.87941
CTGCAAT285200.0127.993522
AGATTGG87400.0126.067221
GATTGGA87400.0125.4233552
TGCAATT333500.0122.739143
TTGGAAG90100.0119.973544
ATTGGAA92800.0117.6562653
GACCGGA67900.0115.209882
AGACCGG70700.0111.435681
CGCAATT46800.0111.228643
CCGGAAG71450.0108.368154
CCGCAAT45650.0107.677992
ACCGGAA72600.0107.2520753
GGAAGAG3576850.0106.4763646
GAAGAGC3567500.0106.025267
CGGAAGA3457000.0105.816265
AAGAGCA3593700.0105.5018848
TCGGAAG3362700.0104.950894
AGAGCAC3606150.0104.643849
ATCGGAA3391000.0104.642893
Produced by FastQC (version 0.11.5)