FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8165456
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT103162412.634003538810326Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT304840.3733288134796146Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT220090.2695379168046463Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT179670.2200367009509328Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT156810.1920407139540033Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT118200.14475615323871685Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC86920.10644843349838637RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC85740.10500332130869361RNA PCR Primer, Index 16 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA799450.0130.524921
CTGCAAT809550.0128.761692
TGCAATT955750.0120.9668963
CGCAATT106650.0117.398723
CCGCAAT100550.0117.382732
ACCGCAA106900.0110.619291
GCAATTT1223700.0105.811554
AATTTCC1316250.0105.031676
CAATTTC1286700.0104.499055
TTTCCAC1337850.0102.583728
ATTTCCA1346500.0102.270527
TTCCACA1389500.098.6974649
GACCGGA45600.086.013042
AGACCGG47550.082.998521
CGGCAAT24150.076.851922
AGATTGG79850.068.448511
ACCGGAA58350.067.9639053
GATTGGA81500.066.538942
GTTCCAC57850.061.2803735
ATTGGAA93950.057.335543
Produced by FastQC (version 0.11.5)