FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2899965
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT54143418.670363263004898Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT165910.5721103530559851Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT116530.40183243590870926Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT96840.3339350647335399Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT84990.2930725025991693Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT43630.15045009163903703Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC35660.12296700132587808RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC33980.11717382795999262RNA PCR Primer, Index 16 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT363050.0134.575122
ACTGCAA364450.0134.333421
TGCAATT429700.0126.7942053
CGCAATT50250.0121.914793
CCGCAAT46450.0120.806692
ACCGCAA49850.0112.685961
AATTTCC618550.0106.7293556
GCAATTT577850.0106.51934
CAATTTC605100.0106.094725
ATTTCCA626600.0105.078717
TTTCCAC627550.0104.3384868
TTCCACA655000.099.97699
CGGCAAT10150.093.570942
GACCGGA21350.093.383582
GATTGGA33800.090.946272
AGACCGG22550.090.233321
AGATTGG34850.088.622021
ACCGGAA23750.083.946933
ATTGGAA38050.080.21643
AGAACGG21850.079.488071
Produced by FastQC (version 0.11.5)