FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12270753
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT3608642.9408464174936944Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1117950.0129.015842
ACTGCAA1128150.0128.335171
TGCAATT1290450.0123.179863
ACCGCAA139550.0122.7741
CCGCAAT143900.0119.3509142
CGCAATT155300.0118.665813
GCAATTT1666350.0107.904224
CAATTTC1762400.0105.9798365
AATTTCC1815750.0105.849856
TTTCCAC1845750.0103.265058
ATTTCCA1877650.0102.0099267
TTCCACA1909650.099.957689
CGGCAAT37400.076.761792
ACGGCAA47450.060.945171
GTTCCAC96000.050.369665
GGCAATT65950.047.8185043
ATGCAAT191250.047.3459132
AGACCGG29850.046.248931
GACCGGA31200.043.2195132
ACGCAAT25100.038.126042
Produced by FastQC (version 0.11.5)