FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5396276
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT152485028.2574501378358Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT454570.8423772245896984Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT329420.6104580269800878Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT266790.4943965060349026Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT232470.4307970904379242Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT133640.24765226982459754Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC104340.19335556594955483RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC101980.18898217956235003RNA PCR Primer, Index 16 (96% over 25bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA86000.15936916495746326No Hit
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC80150.1485283554807056Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT78560.14558187905881761Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA71120.13179459316017195No Hit
AGATCGGAAGAGCACACGTCTTAACTCCAGTCCATCTCGTATGCCGTCTT70600.1308309656511268Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAT59790.1107986322419387No Hit
AGATCGGAAGACACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC56160.10407177097687369RNA PCR Primer, Index 16 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA1066900.0139.923871
CTGCAAT1083200.0137.520972
CCGCAAT141750.0132.511122
TGCAATT1362750.0127.41353
CGCAATT165300.0126.307053
ACCGCAA151700.0123.806421
GCAAGTT66450.0116.818731
GTTCCAC81400.0114.615145
CGGCAAT25950.0114.2599262
AGATTGG69950.0110.869721
GATTGGA69650.0110.642022
AAGTTCC70050.0110.42423
AGTTCCA72150.0107.309674
GACCGGA52200.0104.9927442
AGACCGG53050.0103.990261
GGCAATT32750.0102.046393
ATTGGAA76250.0100.970063
ACTTTCC68850.0100.766453
TTGGAAG76050.0100.56744
CAAGTTC77150.0100.355992
Produced by FastQC (version 0.11.5)