FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4434053
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT61495813.868981719433664Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT181320.40892609989100265Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT130190.2936139915332541Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT105770.23854022493641822Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT94070.21215353086668112Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT54010.12180729459029922Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA492050.0132.76751
CTGCAAT493800.0131.94092
CCGCAAT65500.0125.955382
TGCAATT606550.0122.41483
ACCGCAA67900.0121.6971361
CGCAATT73200.0121.619453
GCAATTT805200.0105.015794
AATTTCC869800.0104.683026
CAATTTC849000.0103.969955
TTTCCAC887450.0101.80758
ATTTCCA892350.0101.7277767
TTCCACA917700.098.648019
CGGCAAT16700.095.93822
GACCGGA23450.093.364092
AGACCGG25300.087.0385741
ACCGGAA25850.084.134963
AGATTGG42250.071.385671
ACGGCAA22650.070.590161
GGCAATT25950.069.004173
AGAACGG28350.067.9332661
Produced by FastQC (version 0.11.5)