FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14071593
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT12400348.812321391046487Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT364450.2589969735480553Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT263740.18742725148460448Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT215050.15282562535741334Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT188280.1338014821776042Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1061250.0125.154852
ACTGCAA1069900.0124.412391
TGCAATT1269250.0118.6158753
CGCAATT158600.0115.692493
CCGCAAT150250.0112.085892
ACCGCAA155000.0108.429881
GCAATTT1651000.0103.355614
AATTTCC1785100.0102.637496
CAATTTC1750300.0101.690735
TTTCCAC1836050.098.954838
ATTTCCA1851250.098.5777057
TTCCACA1918600.094.931449
GACCGGA65100.071.605472
AGACCGG66100.070.5730061
CGGCAAT39650.063.080062
ACCGGAA85150.055.0853163
ACGGAAG74350.052.2631264
AGATTGG123900.051.2560581
GATTGGA127150.049.2621772
GTTCCAC102100.048.992585
Produced by FastQC (version 0.11.5)