FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6922349
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT2989384.318447394085447Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT86360.12475533955309101Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT456850.0127.68392
TGCAATT506800.0126.180423
ACTGCAA469950.0124.367091
CGCAATT59150.0119.076323
CCGCAAT59150.0112.460972
GCAATTT650000.0109.897874
ACCGCAA60750.0109.6509251
AATTTCC695800.0108.04826
CAATTTC682650.0107.295375
TTTCCAC712400.0104.5845648
ATTTCCA730000.0102.579237
TTCCACA736050.0101.5687269
CGGCAAT14650.056.387272
ATGCAAT77850.048.8668752
GACCGGA23650.048.4105222
AGACCGG24200.047.479361
TCCGATC19300.037.5453875
CGTAATT6650.035.958883
ACGGCAA23450.035.352911
TTCCGAT20600.034.4724964
Produced by FastQC (version 0.11.5)