FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5421330
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT2217034.089457752986813Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT63790.11766485345846868Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA459550.0128.104691
CTGCAAT461100.0127.291452
TGCAATT524600.0122.919923
ACCGCAA60300.0121.463761
CCGCAAT61700.0118.499172
CGCAATT66600.0117.614493
GCAATTT701750.0103.919624
AATTTCC756350.0101.676526
CAATTTC738250.0101.245355
TTTCCAC765450.099.738838
ATTTCCA785700.097.537147
TTCCACA793400.096.3622069
CGGCAAT14900.068.0849462
GACCGGA16500.056.212822
AGACCGG16650.055.478421
ACGGCAA18850.053.6337361
GTTCCAC42850.048.0260475
ATGCAAT82100.047.3958662
TCCGATC21700.040.738915
CCGATCT24700.040.4843756
Produced by FastQC (version 0.11.5)