FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5199462
Sequences flagged as poor quality0
Sequence length6
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGG223080.4290443895926156No Hit
GGGGGT110090.21173344472947392No Hit
GGGTGG106880.20555972906427628No Hit
TGGGGG102550.19723194438193797No Hit
GGGGTG100770.19380851326541093No Hit
GTGGGG94200.1811725905487914No Hit
GGGGCG93360.17955703878593593No Hit
GGTGGG90940.17490271108818567No Hit
CGGGGG89460.17205626274410699No Hit
GGGCGG89210.17157544376706668No Hit
GGCGGG88200.1696329350998238No Hit
AGGGGG87820.16890209025472253No Hit
GGGAGG86260.16590177983799093No Hit
GGGGGC81840.15740090032391812No Hit
GCGGGG80700.15520836578861427No Hit
GAGGGG80090.15403516748463592No Hit
GGAGGG73950.1422262534085257No Hit
GGGGGA73360.14109152062271058No Hit
GGGTGT68440.13162900315455714No Hit
GGGGTT68140.13105202038210878No Hit
GGGGAG66000.12693620993864366No Hit
TGGGGT65320.125628382321094No Hit
GGGGTA61950.11914694251059052No Hit
GGTTGG60300.11597353726212442No Hit
TGTGGG59200.11385793376314704No Hit
GGTGGT58330.11218468372304674No Hit
GGGTTG58230.1119923561322306No Hit
TGGGTG56390.10845352846121387No Hit
GTTGGG56390.10845352846121387No Hit
TGGTGG53570.1030298904001991No Hit
AGGGGT53170.10226058003693458No Hit
GTGGGT53160.10224134727785299No Hit
GGGTAG52570.10110661449203784No Hit
GGGCCG52340.10066426103316074No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)