FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9287369
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT5031275.417325401844161Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT145630.15680436515443716Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT98050.10557349449558857Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT620350.0123.281172
ACTGCAA633350.0121.274461
TGCAATT715700.0118.1358953
ACCGCAA83250.0113.057461
CCGCAAT84550.0111.244252
CGCAATT89450.0111.226093
GCAATTT940450.0101.4536364
AATTTCC1020750.098.49156
CAATTTC993850.098.284615
TTTCCAC1027450.097.059348
ATTTCCA1054900.095.000827
TTCCACA1070350.093.277499
GACCGGA32300.059.4510882
AGACCGG33900.056.8584941
CGGCAAT20850.052.479222
ACCGGAA42650.044.3443153
ATGCAAT128000.042.7418632
AGAACGG40250.041.2019841
CGGAAGT33650.040.9152345
TCCGATC34300.039.0835575
Produced by FastQC (version 0.11.5)