FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6407797
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT92036714.363235913996652Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT97400.15200231842550568Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT87270.13619345306975236Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC83540.13037241972553126RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC78020.12175791461558473RNA PCR Primer, Index 16 (96% over 25bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT72680.11342431728096256Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA70490.11000660601451638No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA903750.0136.738461
CTGCAAT899200.0136.652652
CCGCAAT131050.0131.612582
TGCAATT1029300.0128.982793
CGCAATT141000.0128.916443
ACCGCAA135300.0128.047421
GCAATTT1422800.0106.668134
AATTTCC1449350.0105.736596
CAATTTC1447050.0105.422785
TTTCCAC1444000.0104.7678458
ATTTCCA1467450.0103.622247
TTCCACA1470850.0103.067279
CGGCAAT20250.082.519482
ACGGCAA26500.063.6215861
ATTGCAA63050.063.2775461
GGCAATT27800.061.4134033
ATGCAAT112900.051.5456772
TATGCAA40650.047.3748361
GATTGGA42850.045.779032
AGATTGG43800.045.1291471
Produced by FastQC (version 0.11.5)