FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2975594
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT92672431.144168189611886Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT312891.0515211416611272Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT286760.9637067422504548Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT220530.7411293341766384Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT220220.7400875253814869Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT107430.36103715762298216Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT66540.223619216868968Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC62550.2102101294733085RNA PCR Primer, Index 16 (96% over 25bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT59410.19965761458048378Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC58280.19586005348847996RNA PCR Primer, Index 16 (96% over 25bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT53320.17919111276605612Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCTTATGCCGTCTT52490.17640175373387632Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC47500.15963199280547008Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGGAAGAGCACACGTCTGAACGCCAGTCCATCTCGTATGCCGTCTT42230.1419212432878948Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT38360.1289154367161649Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC34050.11443093379002647Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGACACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC33070.11113747372793466RNA PCR Primer, Index 16 (100% over 22bp)
AGATCGGAAGATCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT33020.11096944005129733Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTAGTATGCCGTCTT31510.10589482301684974Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC30860.10371038522056436TruSeq Adapter, Index 16 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT241050.0125.210652
ACTGCAA240350.0124.4920961
GACCGGA45600.0114.92412
TGCAATT291550.0114.528513
AGACCGG45400.0113.891261
ACCGGAA47250.0111.670383
AGATTGG51550.0107.19711
GATTGGA51600.0106.758282
CGCAATT37850.0104.744183
CCGCAAT35950.0103.432292
CCGGAAG51950.0102.26844
AATTTCC413050.0102.194056
CAATTTC397800.0101.472915
GCAATTT373850.0101.203644
ATTGGAA54500.0100.804793
ATTTCCA420900.099.9608467
TTGGAAG55600.098.944124
ACGGAAG38650.098.641424
TTTCCAC428850.097.8525858
TGATCGG12350.095.7144241
Produced by FastQC (version 0.11.5)