FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005483252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7792465
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT328665542.177346962739Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT1178921.5128973951118163Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT1045721.3419630373700748Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT753490.9669469160272134Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT728740.9351854644197953Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT662650.8503727639456834Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT590600.7579116492663105Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAGCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT458310.5881450863109426Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT306810.3937265037443223Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT251360.3225680192339651Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC228510.29324482047721745RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC225330.28916395517977944RNA PCR Primer, Index 16 (96% over 25bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT201060.25801848323989907Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC180810.23203184101564783Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGGAAGACACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC124550.15983389081632066RNA PCR Primer, Index 16 (100% over 22bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC122190.15680532411759307Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC109880.1410080122271964TruSeq Adapter, Index 16 (95% over 22bp)
AGATCGGAAGAGCAACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC98470.1263656622134331TruSeq Adapter, Index 16 (95% over 22bp)
AGATAGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT92850.11915356693934462Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGCACAGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC83880.10764244690223182TruSeq Adapter, Index 16 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)