FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7364248
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT196609626.697851566106955Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT553600.7517400283097473Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT388850.5280240426449516Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT321050.4359576157674212Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT301620.40957338753393424Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT172910.23479654677571968Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC156080.21194288948443887RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC154400.20966159749101335RNA PCR Primer, Index 16 (96% over 25bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA145090.1970194376941135No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA120490.16361480493323963No Hit
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC119780.16265068748363715Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAT103090.13998713785847516No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCATTT88430.12008014939203569No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAGA85910.11665821140189737No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAG83670.11361648874399666No Hit
AGATCGGAAGACACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC82750.11236720979521603RNA PCR Primer, Index 16 (100% over 22bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGGAAA79070.10737009400009342No Hit
NGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT78500.10659608421660977Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC77270.10492585257856607TruSeq Adapter, Index 16 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA1807750.0141.111391
CTGCAAT1806000.0140.948232
CCGCAAT240500.0135.192492
TGCAATT2085600.0133.337273
CGCAATT262350.0131.031343
ACCGCAA254650.0127.6992951
CGGCAAT40850.0114.59142
AGATTGG89350.0112.090011
AATTTCC3005750.0110.5851756
CAATTTC2939450.0110.09665
GCAATTT2878000.0109.806184
ATTTCCA3019250.0109.6371157
GCAAGTT73150.0109.471351
TTTCCAC3035100.0108.632438
GATTGGA91750.0108.436642
GTTCCAC85550.0106.5545965
TTCCACA3142400.0104.978439
GGCAATT47250.0102.4439243
AAGTTCC78700.0101.4655153
ATTGGAA98150.0101.218233
Produced by FastQC (version 0.11.5)