FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11074973
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT181128016.354712557764252Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT522060.4713871537203747Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT363260.32800079964077566Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT306420.27667787542235994Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT284840.25719250060474186Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT159530.14404549789873078Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC145690.13154885343738537RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC144610.1305736817597659RNA PCR Primer, Index 16 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1073150.0133.716482
ACTGCAA1110650.0129.705521
TGCAATT1202350.0128.906253
CGCAATT150500.0124.318033
CCGCAAT147600.0119.936522
ACCGCAA160950.0110.084261
GCAATTT1593350.0110.043594
AATTTCC1695850.0108.874066
CAATTTC1659000.0108.327125
TTTCCAC1718400.0106.433298
ATTTCCA1743200.0105.517697
TTCCACA1785350.0102.61669
GACCGGA72000.087.559072
AGACCGG73400.086.706351
AGATTGG123150.079.201471
GATTGGA124600.077.813052
ACCGGAA84750.074.813973
ATTGGAA139750.069.066393
CGGCAAT34050.068.738462
AGAACGG77950.065.969351
Produced by FastQC (version 0.11.5)