FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8492247
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT90578010.665963908021045Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT258750.3046896775376411Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT180690.21277054235469128Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT152850.17998769936861234Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT141920.16711713637156336Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT856500.0131.791812
ACTGCAA867800.0130.477831
TGCAATT973150.0126.686623
CGCAATT124150.0122.567893
CCGCAAT116750.0121.895792
ACCGCAA120600.0118.332241
GCAATTT1273300.0109.9405754
AATTTCC1355850.0108.543146
CAATTTC1329600.0107.901225
TTTCCAC1365950.0106.8175358
ATTTCCA1390250.0105.403927
TTCCACA1415450.0103.225339
GACCGGA41600.079.428362
AGACCGG43600.076.7401661
CGGCAAT27150.070.726172
ACCGGAA54800.060.2959863
GATTGGA80150.057.137032
AGATTGG81000.056.4829031
ACGGCAA33900.056.0005341
ACGGAAG49050.051.8528024
Produced by FastQC (version 0.11.5)