FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13651351
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT4447873.258190343212185Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT952700.0127.646232
TGCAATT1048000.0124.926523
ACTGCAA986150.0124.220021
CGCAATT125950.0120.172893
CCGCAAT125200.0115.6244052
ACCGCAA130600.0111.168271
GCAATTT1366850.0107.8545764
AATTTCC1459050.0105.604876
CAATTTC1434800.0104.6417165
TTTCCAC1501200.0101.586698
ATTTCCA1529800.0100.3366857
TTCCACA1571700.097.4173369
CGGCAAT33250.055.157512
ACGGCAA44450.041.850121
TCCGATC57900.041.815965
CCGATCT66150.039.8881726
TTCCGAT59950.039.6604164
GTTCCAC104650.037.6127245
ATGCAAT148050.036.6242642
GACCGGA34800.036.2448042
Produced by FastQC (version 0.11.5)