FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6591107
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT75812111.502180134535822Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT217150.3294590726565355Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT125660.19065082693999658Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT109950.16681568058294305Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT103230.15662012466191186Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC67170.10191004333566425Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT721600.0133.230582
ACTGCAA729450.0132.441681
TGCAATT799400.0127.427623
CCGCAAT93000.0124.2407462
CGCAATT97150.0122.664163
ACCGCAA98550.0116.970521
GCAATTT1039400.0110.605274
AATTTCC1101100.0109.227176
CAATTTC1076300.0108.706565
TTTCCAC1111450.0107.099698
ATTTCCA1130250.0106.089457
TTCCACA1153300.0103.515039
GACCGGA29150.084.049822
AGACCGG29750.082.82141
CGGCAAT20750.080.346762
AGATTGG58150.064.3705751
GATTGGA58600.063.5805362
ACGGCAA27250.061.6134221
ACCGGAA40450.060.39073
ACGGAAG34600.058.2403684
Produced by FastQC (version 0.11.5)