FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15601604
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT8819425.652893125604265Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT251810.16140007142855312Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1273250.0129.98512
ACTGCAA1308000.0127.156411
TGCAATT1405950.0125.140753
CGCAATT178500.0120.289523
CCGCAAT173100.0118.5145652
ACCGCAA177300.0116.050011
GCAATTT1822800.0109.657654
AATTTCC1936200.0107.843616
CAATTTC1901950.0106.904615
TTTCCAC1968250.0104.930078
ATTTCCA2004200.0103.674647
TTCCACA2040150.0101.523429
CGGCAAT45350.070.013082
GACCGGA51150.054.9880682
ACGGCAA58200.054.572331
AGACCGG53600.054.509891
TCCGATC87200.051.37485
CCGATCT100500.050.3461386
TTCCGAT88900.049.576974
GGCAATT76200.043.0947343
Produced by FastQC (version 0.11.5)