FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5491030
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT3799986.92034099249139Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT129670.2361487735452183Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT117980.21485950723270497Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT90780.1653241741531188Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT90420.1646685594505949Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA327650.0114.754511
CTGCAAT340900.0111.5045852
TGCAATT434800.095.092673
ACCGCAA45000.094.7536541
CCGCAAT45700.094.6924062
CGCAATT50000.092.057843
AATTTCC583800.088.266026
GCAATTT527900.088.201374
CAATTTC566500.087.127815
GACCGGA26750.085.8998642
TTTCCAC604050.084.995028
ATTTCCA609750.084.40187
AGACCGG27650.082.8749161
TTCCACA632250.081.144499
ACCGGAA33750.065.935863
TGATCGG10600.060.88571
CGGCAAT13550.055.100432
AGAACGG31050.053.4816781
ACGGAAG31350.053.4067154
GAACGGA32450.052.0473022
Produced by FastQC (version 0.11.5)