FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5857229
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT3513835.999133720057727Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT121210.20694085889419725Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT109660.18722163671592829Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT85560.14607590039590393Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT85510.1459905357977296Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA314800.0110.641581
CTGCAAT323300.0109.295922
CCGCAAT41350.096.760622
ACCGCAA40750.096.432131
TGCAATT416300.092.2263263
CGCAATT46200.090.2105263
GCAATTT499550.086.186424
AATTTCC557950.086.024616
CAATTTC541250.084.929725
TTTCCAC574200.083.362138
ATTTCCA587000.081.767357
GACCGGA25400.081.614522
AGACCGG25700.081.247391
TTCCACA595000.080.433369
ACCGGAA32200.064.82883
AGAACGG29400.051.787151
ACGGAAG30300.051.431264
GAACGGA30550.051.2480772
TGATCGG10300.046.45761
AGATTGG53700.046.309481
Produced by FastQC (version 0.11.5)