FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2367946
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT1924758.128352589121542Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT66700.28167872071407035Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT59030.24928777936659027Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT46880.1979774876623031Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT46710.1972595658853707Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT203150.0130.636812
ACTGCAA206100.0127.3273541
TGCAATT231400.0125.80143
CGCAATT28950.0122.4377753
CCGCAAT29150.0115.388372
GCAATTT291800.0112.999524
AATTTCC326050.0112.323586
CAATTTC313450.0111.926315
ACCGCAA30300.0110.3238451
ATTTCCA338000.0108.073567
TTTCCAC339100.0107.402318
TTCCACA356950.0102.071719
GACCGGA12050.077.002632
AGACCGG12550.072.8010941
GTTCCAC19100.070.9681245
ACGGAAG14700.064.5951464
GAACGGA14650.064.326272
ACCGGAA14850.062.9677733
CGGCAAT6650.061.044852
AACGGAA16450.057.724543
Produced by FastQC (version 0.11.5)