FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2222459
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT79521935.78104252991844Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT124320.5593803980185911Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC115700.520594530652759Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT107230.4824835913733391Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT86820.3906483764154929Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT81620.3672508694198633Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGT77010.34650807956412244Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT54820.24666371798084913Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC45770.20594305676730146RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC45650.20540311429817154RNA PCR Primer, Index 16 (96% over 25bp)
CTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC36930.16616729487473111Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTA35280.15874308592419478Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC35240.15856310510115149Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT33840.1522637762946358Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCG31990.14393966322888296Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCAGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT26690.12009220417564509Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGACACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC24220.10897838835272101RNA PCR Primer, Index 16 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT265250.0135.469542
ACTGCAA265200.0135.440861
TGCAATT302450.0129.996673
CCGCAAT32400.0122.183732
CGCAATT35300.0120.5617753
GCAATTT419850.0104.629234
AATTTCC435000.0104.451986
CAATTTC430300.0103.688935
ATTTCCA442150.0102.915097
TTTCCAC445000.0101.97678
ACCGCAA39450.0100.0823751
TTCCACA456050.099.489929
AGACCGG20900.096.539321
GACCGGA20900.096.4415742
GATTGGA22800.083.3167342
AGATTGG23450.082.3274151
CCGGAAG25100.080.589174
CGGAAGA1708100.079.852965
ACCGGAA25600.079.581583
GGAAGAG1736050.079.544576
Produced by FastQC (version 0.11.5)