FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15205484
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT12825488.434772612302245Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT195170.12835500665417818Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT172570.11349194803664257Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT775800.0120.721912
TGCAATT843050.0119.541073
ACTGCAA827450.0113.12331
GCAATTT1056900.0106.116074
CGCAATT97900.0105.9772343
CAATTTC1115900.0101.8627245
AATTTCC1135600.0101.6259166
TTTCCAC1178550.097.798048
CCGCAAT101550.097.174522
ATTTCCA1198800.096.371717
TTCCACA1210100.095.3800359
ACCGCAA113100.087.009871
GACCGGA53600.049.780272
AGACCGG57700.047.0399931
CGGCAAT33050.045.4122622
TCCGATC69100.044.907175
TTCCGAT67350.044.2442674
CCGATCT75950.041.047066
CGGAAGA5419300.040.0631945
AGAGCAC5304350.039.7833149
Produced by FastQC (version 0.11.5)