FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5898595
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT1030001.7461785391266904Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT533600.0132.878072
ACTGCAA541450.0131.309481
TGCAATT603850.0129.456193
CGCAATT70700.0122.3414463
CCGCAAT66100.0120.77042
ACCGCAA67650.0118.227751
GCAATTT794550.0110.366484
CAATTTC822500.0108.4486165
AATTTCC838850.0108.214436
TTTCCAC857200.0105.669548
ATTTCCA872550.0104.134637
TTCCACA881800.0102.828519
CGGCAAT16400.071.179792
ATGCAAT74450.058.0438462
ACGGCAA22350.051.6327481
GGCAATT23750.050.675383
TCCGATC25300.049.576345
ATTGCAA42850.043.8686831
CCGATCT30700.042.979716
ACGCAAT9700.041.1117132
Produced by FastQC (version 0.11.5)