FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10893412
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT200811418.434205921891138Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT303600.2787005577315904Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT265240.24348661374416022Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT209080.1919325184799767Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT204780.18798517856480595Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC184240.16912974557466476Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT130630.11991651467878016Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC113110.10383339948952634RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC112530.10330096759399168RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGT111060.10195152813461934Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT723000.0129.037142
ACTGCAA745950.0125.421881
TGCAATT800300.0124.696853
CGCAATT97000.0112.942233
CCGCAAT96850.0108.861492
GCAATTT1047900.0106.864074
AATTTCC1108050.0104.409876
CAATTTC1092550.0104.173355
TTTCCAC1130250.0102.105638
ATTTCCA1155500.0100.18377
TTCCACA1168650.099.004959
ACCGCAA115900.091.299951
GACCGGA59600.083.340062
AGACCGG66150.075.4840241
ACCGGAA80850.061.160073
CGGAAGA5694650.060.248985
TCGGAAG5669700.059.7418254
ATCGGAA5735700.059.621963
AGAGCAC5599050.059.4429369
GAAGAGC5671950.059.429067
Produced by FastQC (version 0.11.5)