FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8218902
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT122463914.90027500023726Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT196590.2391925344772331Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT171670.208872182683283Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT137330.16709044590141117Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT131670.16020388124837112Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT106680.12979835992690994Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA106580.12967668917332267No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA88740.10797062673335196No Hit
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC88670.10788545720584089RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC86650.10542770798337782RNA PCR Primer, Index 16 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA1345650.0136.7661
CTGCAAT1395500.0131.535552
CCGCAAT170100.0125.111972
TGCAATT1728600.0120.510293
ACCGCAA179750.0118.4810261
CGCAATT197950.0117.75473
AATTTCC2469850.097.802056
GCAATTT2401200.097.458034
CAATTTC2443300.097.2345665
ATTTCCA2504200.096.5757147
TTTCCAC2510250.096.064528
TTCCACA2569900.093.992759
CGGCAAT30400.092.0680162
GACCGGA40650.078.485092
ATTGCAA92250.074.174561
AGACCGG43500.073.896541
GGCAATT44850.071.9366763
GTTCCAC57700.070.491145
ACGGCAA41050.069.291911
AGATTGG63900.063.818281
Produced by FastQC (version 0.11.5)