FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11393924
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT147212612.920272243346542Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT230450.20225692219818212Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT195480.17156512541245666Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT158490.13910045389103876Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT154050.13520364011555633Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT128150.11247222642524209Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1159700.0128.281022
ACTGCAA1166250.0127.94081
TGCAATT1400400.0119.66163
CCGCAAT149400.0117.403062
CGCAATT164050.0114.160943
ACCGCAA156550.0111.9954451
GCAATTT1857150.0101.444034
AATTTCC1936700.0100.373076
CAATTTC1910650.0100.128125
TTTCCAC1977900.098.1317758
ATTTCCA1991800.097.7338647
TTCCACA2024300.096.125989
CGGCAAT33750.075.409752
GACCGGA48150.067.012822
AGACCGG50700.063.9817431
ACGGCAA47700.053.85691
ACCGGAA64800.050.2389683
ATTGCAA111300.049.227551
GGCAATT57350.048.926813
AGATTGG89550.047.1643561
Produced by FastQC (version 0.11.5)