FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7867953
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT155713419.790840133386663Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT241870.30741159740023866Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT205030.2605887452555957Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT163290.20753809790170327Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT162850.20697886731148493Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA121670.15463996798150675No Hit
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT111080.1411803044578431Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA100780.12808922473227788No Hit
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC93120.1183535285480226RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC92930.11811204261133741RNA PCR Primer, Index 16 (96% over 25bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAT84450.10733414396349343No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1465650.0138.89392
ACTGCAA1481750.0137.992871
CCGCAAT175450.0132.702962
TGCAATT1691700.0131.090413
CGCAATT195000.0126.531583
ACCGCAA191100.0122.1345141
AATTTCC2365250.0108.835036
GCAATTT2289200.0108.737334
CAATTTC2329450.0108.630545
ATTTCCA2395650.0107.482847
CGGCAAT29150.0107.209292
TTTCCAC2400000.0106.943968
TTCCACA2451950.0104.885099
GGCAATT38200.086.1715853
GACCGGA44250.077.834842
AGACCGG44400.077.6414641
ACGGCAA41600.074.667821
AGATTGG54250.073.577671
GTTCCAC44600.072.990535
ATTGCAA64700.072.686511
Produced by FastQC (version 0.11.5)