FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10997038
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT4184013.8046699483988324Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT817150.0128.482992
ACTGCAA842600.0125.302681
TGCAATT915150.0124.3577043
CGCAATT97000.0118.342433
CCGCAAT97150.0112.421922
ACCGCAA100650.0109.330511
GCAATTT1173750.0107.60414
AATTTCC1246050.0105.602876
CAATTTC1217350.0105.213425
TTTCCAC1268800.0103.1551068
ATTTCCA1298550.0101.020727
TTCCACA1323100.099.042189
CGGCAAT25300.059.6227042
ACGGCAA32400.046.1202661
ATGCAAT123600.040.2507972
TTCCGAT36800.036.645564
TCCGATC36850.036.5951735
ATTGCAA85150.035.8646851
CCGATCT43850.034.389696
GGCAATT47750.034.321513
Produced by FastQC (version 0.11.5)