FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11778413
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT6319265.36512007177877Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT160400.1361813344463299Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1018850.0132.164572
ACTGCAA1047250.0129.1581
TGCAATT1138350.0127.4794543
CGCAATT127850.0121.610563
CCGCAAT125850.0117.323412
ACCGCAA129350.0114.618281
GCAATTT1503600.0107.9494
AATTTCC1590800.0106.497826
CAATTTC1556500.0105.834195
TTTCCAC1627650.0103.516568
ATTTCCA1647750.0102.601427
TTCCACA1686550.0100.069079
CGGCAAT31700.070.918992
TCCGATC46950.050.9595955
ACGGCAA45600.048.9920161
ATGCAAT129900.047.676992
GGCAATT50450.046.2851753
CCGATCT56600.045.3441856
TTCCGAT51950.044.380954
GTTCCAC84200.044.344815
Produced by FastQC (version 0.11.5)