FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11719919
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT297872925.415952106836233Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT752970.642470310588324Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT429450.3664274471521518Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT371520.3169987778925776Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT262750.2241909692379273Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT185930.15864444114332188Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC173950.14842252749357737RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC173780.14827747529654428RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC153910.13132343320802814Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC128500.10964239599266855Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT627100.0125.129712
TGCAATT704800.0121.0283053
ACTGCAA674350.0116.91111
CGCAATT84150.0107.3738863
GCAATTT913850.0104.343744
AATTTCC976300.0102.121536
CAATTTC952500.0101.6007845
TTTCCAC993700.099.815338
CCGCAAT86050.099.449162
ATTTCCA1016300.097.9238367
TTCCACA1044850.095.01229
AGATTGG142800.085.640841
GACCGGA75950.084.983032
GATTGGA143350.084.587972
AGACCGG79550.081.243581
ATTGGAA154500.078.477033
ACCGCAA110850.077.018011
AGAGCAC6560650.075.449429
GAAGAGC6626250.075.343637
AAGAGCA6600100.075.2719658
Produced by FastQC (version 0.11.5)