FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7179581
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT3023994.2119310305155695Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT77050.107318240437708Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT720900.0131.70662
ACTGCAA725650.0131.650621
TGCAATT814200.0126.2617953
CCGCAAT79750.0119.8564152
CGCAATT85850.0119.4423753
ACCGCAA82800.0115.990381
GCAATTT1050550.0108.760344
AATTTCC1115700.0107.16686
CAATTTC1082600.0107.158255
TTTCCAC1120750.0106.088798
ATTTCCA1147800.0103.866537
TTCCACA1158100.0102.955279
CGGCAAT20800.072.174472
ACGGCAA26450.056.2202151
GGCAATT32350.048.196283
ATGCAAT97100.048.0997922
GTTCCAC51700.045.9970325
TCCGATC24500.042.9095
ATTGCAA56100.042.669291
GACCGGA20150.039.950712
Produced by FastQC (version 0.11.5)