FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13436534
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT10274337.64656272220202Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT253220.188456338517061Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT142040.10571178549468188Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT968150.0127.221542
TGCAATT1064100.0123.850423
ACTGCAA1002800.0123.353581
CGCAATT122700.0115.3097463
CCGCAAT120100.0111.293862
GCAATTT1361600.0108.0185554
AATTTCC1451550.0105.815846
CAATTTC1420650.0104.991055
ACCGCAA129300.0103.343191
TTTCCAC1479500.0103.292498
ATTTCCA1508550.0101.6446157
TTCCACA1536400.099.674719
CGGCAAT34900.050.91312
GACCGGA48300.047.7495042
AGACCGG50100.045.899961
GATTGGA113000.042.937482
AGATTGG117950.041.3911861
TTCCGAT69300.041.1159974
TCCGATC70050.040.5713775
CCGATCT77650.040.3344356
Produced by FastQC (version 0.11.5)