FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3854848
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT123358432.00084672599283Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT456121.183237315712578Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT401881.0425313786691461Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT287100.7447764477354231Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT280600.7279145636870766Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT254530.6602854379731704Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT220920.5730965267631825Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAGCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT168010.4358407906096427Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT117720.3053816907955904Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT101210.26255250531279056Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC94230.24444543598087395RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC91970.23858268860406429RNA PCR Primer, Index 16 (96% over 25bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT77790.2017978400185948Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC73920.19175853366981008Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGGAAGACACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC50000.12970680037189533RNA PCR Primer, Index 16 (100% over 22bp)
AGATCGGAAGAGACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC45100.11699553393544959TruSeq Adapter, Index 16 (95% over 22bp)
AGATCGGAAGAGCAACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC39970.10368761621729312TruSeq Adapter, Index 16 (95% over 22bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC39080.10137883517067339Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT404850.0136.357382
ACTGCAA404650.0134.802151
TGCAATT470050.0131.047993
CCGCAAT47850.0120.1575852
CGCAATT52550.0119.06883
CAATTTC707600.0115.074545
AATTTCC747000.0113.7302256
GCAATTT610150.0112.789784
AGATTGG68150.0112.169691
GACCGGA64250.0112.169082
GATTGGA68450.0111.959622
AGACCGG63450.0111.905611
ATTTCCA757600.0111.673827
TTTCCAC771450.0109.264838
TCAATTT76400.0108.847564
TTCAATT69650.0108.469233
ATTGGAA72450.0106.779023
ACCGGAA68650.0106.564013
TTCCACA814750.0103.841899
TTGGAAG74800.0103.5185854
Produced by FastQC (version 0.11.5)