FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10824961
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT120306811.113832188402343Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT441710.40804765947886557Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT391340.36151631400796735Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT282640.26110024784384905Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT273210.2523889000616261Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT254620.23521562802859058Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT220940.2041023519622842Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAGCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT170250.1572753934171218Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT114290.10558005705517091Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT582400.0123.2226942
TGCAATT657000.0122.008833
ACTGCAA598300.0118.163011
CGCAATT76250.0109.349453
CAATTTC978850.0109.105145
GCAATTT826100.0108.7430044
AATTTCC1033200.0108.555166
TTTCCAC1072200.0103.975828
ATTTCCA1082050.0103.1949167
CCGCAAT74700.0102.981552
TTCCACA1139900.098.163559
GACCGGA73250.093.142232
ACCGCAA84750.089.168821
AGACCGG76550.087.825291
ACCGGAA90750.077.097323
ACATCGG50700.076.9589841
TCAATTT162550.068.9134754
GATTGGA119550.067.561652
CATCGGA58900.067.457442
AGATTGG119500.066.69831
Produced by FastQC (version 0.11.5)