FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4153921
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT112259927.025044530216153Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT182520.43939208280561903Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT176670.4253090032285159Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT169690.40850560229720306Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT111890.2693599613473631Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT96090.23132360966903318Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC88410.21283505391652852Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC78100.18801513076440307RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC76740.18474111568323037RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC59960.14434554725523185Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC45050.10845174956384582Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGACACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC41750.10050744826394147RNA PCR Primer, Index 16 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT449300.0131.2242
ACTGCAA452150.0131.075181
TGCAATT546100.0123.56193
CGCAATT70550.0120.8529743
CCGCAAT66300.0118.1034242
ACCGCAA72550.0107.770681
AATTTCC796700.0101.489956
CAATTTC783750.0101.1965945
GCAATTT758350.0101.139774
ATTTCCA808200.099.649947
TTTCCAC813500.098.6876148
TTCCACA837800.096.240589
GACCGGA33650.091.1396942
ACCGGAA35050.086.87773
AGACCGG35650.086.4235461
GATTGGA37950.084.633872
AGATTGG38750.083.447651
AGAACGG38200.081.605551
ACGGAAG38500.080.222454
GAACGGA40600.076.609662
Produced by FastQC (version 0.11.5)