FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12044840
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT125122410.388049986550257Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT192120.1595039867694382Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT182950.1518907681629644Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT175460.14567233769813465Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT122240.10148744192533898Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT869100.0125.1535642
ACTGCAA879200.0123.7789841
TGCAATT1037700.0120.354923
CGCAATT137700.0116.256263
CCGCAAT131450.0111.30752
ACCGCAA136600.0107.4971
GCAATTT1365650.0104.019024
AATTTCC1456300.0102.3495946
CAATTTC1433450.0102.034745
TTTCCAC1497850.098.872268
ATTTCCA1506200.098.673567
TTCCACA1542800.096.024489
GACCGGA52100.066.520272
AGACCGG53200.065.0093841
ACCGGAA62700.055.5040783
CGGCAAT30150.055.310092
ATGCAAT178900.054.0236172
GAACGGA67900.046.5565262
ACGGAAG68200.046.456694
AGAACGG71400.044.5714341
Produced by FastQC (version 0.11.5)