FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7922054
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT6799098.58248378513956Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT110580.13958501166490409Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT105080.13264236774957605Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT102610.12952448948214693Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT658800.0128.124712
ACTGCAA669750.0126.487831
TGCAATT794750.0120.656693
CGCAATT101900.0116.829463
CCGCAAT95300.0114.8035352
ACCGCAA95850.0113.5585561
GCAATTT1066050.0102.029324
AATTTCC1130300.0101.1777656
CAATTTC1117100.0100.460025
TTTCCAC1159550.097.765248
ATTTCCA1171850.097.200567
TTCCACA1198650.094.654769
GACCGGA30150.071.661832
AGACCGG31950.067.0890051
CGGCAAT21100.064.943882
ACCGGAA40150.054.173783
ACGGCAA26150.052.5158351
ATGCAAT129450.050.6320232
AGAACGG38100.047.1055561
ACGGAAG39600.043.2086074
Produced by FastQC (version 0.11.5)