FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11164750
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT2567662.2997917553012828Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA1046400.0128.660581
CTGCAAT1052750.0127.518922
TGCAATT1269900.0120.589773
ACCGCAA145150.0120.289571
CGCAATT155850.0119.8350753
CCGCAAT146350.0118.7980042
GCAATTT1684250.0102.670334
AATTTCC1782300.0101.790046
CAATTTC1761000.0101.350235
ATTTCCA1831500.098.782067
TTTCCAC1834800.098.183368
TTCCACA1885150.095.8032769
CGGCAAT29400.067.0770952
ATGCAAT199400.057.122042
ACGGCAA38350.050.5867921
ATTGCAA96950.049.9884031
TATGCAA80500.045.2202641
CATGCAA99900.042.184581
GGCAATT52550.040.010153
ACGCAAT23150.039.148192
Produced by FastQC (version 0.11.5)