FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11384576
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT4818424.232410587798791Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA1281100.0133.446261
CTGCAAT1294050.0131.816042
ACCGCAA176700.0127.728491
CCGCAAT179550.0125.5417252
TGCAATT1517550.0123.809323
CGCAATT194700.0122.4737553
GCAATTT2015350.0106.1499254
AATTTCC2129900.0105.087586
CAATTTC2098650.0104.925125
ATTTCCA2178100.0102.365517
TTTCCAC2177050.0102.038328
TTCCACA2228700.099.8817759
CGGCAAT35300.080.307262
ACGGCAA46700.060.9946981
ATGCAAT210700.053.8176772
GGCAATT61100.048.769213
GACCGGA31600.048.1820142
AGACCGG32750.046.5940931
ATTGCAA100650.045.916231
CATGCAA103400.039.9163361
Produced by FastQC (version 0.11.5)