FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10682407
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT4806634.499575797851552Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA1120500.0130.722351
CTGCAAT1120550.0130.244612
ACCGCAA152750.0125.1980061
CCGCAAT153000.0124.016972
TGCAATT1294250.0123.776013
CGCAATT162600.0122.7124253
GCAATTT1733000.0105.0740054
AATTTCC1828750.0104.2078256
CAATTTC1801800.0104.004615
TTTCCAC1862000.0101.4529658
ATTTCCA1875450.0101.179087
TTCCACA1924750.098.424169
CGGCAAT32550.070.166262
ACGGCAA36450.063.7889141
TCCGATC33050.047.162765
ATGCAAT190550.045.5844462
GACCGGA29900.044.3760572
TTCCGAT35500.042.072624
CCGATCT38800.040.73386
ATTGCAA94800.040.391971
Produced by FastQC (version 0.11.5)