FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4890006
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT123502425.256083530367857Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT202070.4132305768131982Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT197330.4035373371730014Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT189440.38740238764533214Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT127740.2612266733415051Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT126430.2585477400232229Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC96010.19633922739563098RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC94790.19384434293127656RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC71720.14666648670778729Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA51140.10458064877630006No Hit
AGATCGGAAGACACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC50990.10427390068642042RNA PCR Primer, Index 16 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT649550.0135.896422
ACTGCAA653950.0135.126741
TGCAATT783900.0127.73423
CCGCAAT95500.0123.064082
CGCAATT102750.0122.986743
ACCGCAA103600.0113.474841
AATTTCC1145000.0104.339896
GCAATTT1092500.0104.109144
CAATTTC1128250.0103.944055
ATTTCCA1162650.0102.3745047
TTTCCAC1173750.0101.0769358
TTCCACA1205900.098.50249
GACCGGA37750.086.810672
AGACCGG38400.085.902431
CGGCAAT17250.079.8575742
AGAACGG44900.077.1885451
AGATTGG44400.075.766781
GATTGGA43800.075.316292
GAACGGA45400.075.217122
ACCGGAA43950.074.892743
Produced by FastQC (version 0.11.5)