FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6092341
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT84961413.94560810040016Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT137910.2263661866596108Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT135550.22249247046414508Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT131820.21637002918910808Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA90410.14839944120002474No Hit
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT85260.13994620458703805Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT82990.13622021485665364Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA72740.11939581188905875No Hit
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC65250.1071016871839577RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC64040.1051155869311977RNA PCR Primer, Index 16 (96% over 25bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAT62330.10230878409465262No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA1088300.0138.068621
CTGCAAT1098850.0136.13762
CCGCAAT150400.0130.929062
ACCGCAA154100.0128.162481
TGCAATT1309700.0127.6318743
CGCAATT165400.0127.074883
CGGCAAT22850.0107.246872
AATTTCC1873650.0106.411666
CAATTTC1845550.0106.271995
GCAATTT1796150.0105.7763444
ATTTCCA1895300.0104.659737
TTTCCAC1903450.0103.832838
TTCCACA1956800.0101.235379
GGCAATT31750.082.663133
ACGGCAA31550.077.845121
GACCGGA31350.075.624822
AGACCGG31400.075.208651
ACTTTCC66950.071.5810243
ATTGCAA61750.070.603851
CACTTTC69850.068.091292
Produced by FastQC (version 0.11.5)